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axio imager a2 positive fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axio imager a2 positive fluorescence microscope
    Axio Imager A2 Positive Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1058 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/positive+fluorescence+microscope/Microscope+Axio+Imager%2EA2/pm40816688-75-14-13
    Average 97 stars, based on 1058 article reviews
    axio imager a2 positive fluorescence microscope - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy.
    Article Snippet: .. After 4% paraformaldehyde fixation, DAPI was added for 10min of nuclear staining, and a positive fluorescence microscope (Axio Imager D2, Carl Zeiss, Germany) was utilized to observe the sections and take photos. .. HSC-T6 cells (CL-0116, Procell) were classified as four groups following as control group, TGF-β (80116-RNAH, SinoBiological) treatment group (48 h of 5 ng/ml TGF-β treatment), TGF-β+mimic NC group (24 h of 5 ng/ml TGFβ treatment and then 24 h of mimic NC transfection), and TGF-β +miR125a-5p group(5 ng/ml TGF-β for 24 h, and transfected miR-125a-5p mimic (miR10000829-1-5, Ribobio) for 24 h.) Lipo RNAi MAX was used for transfection (13778-075, Thermo Fisher), and the method was carried out according to our previous paper [8].

    Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy
    Article Snippet: .. After 4% paraformaldehyde fixation, DAPI was added for 10 min of nuclear staining, and a positive fluorescence microscope (Axio Imager D2, Carl Zeiss, Germany) was utilized to observe the sections and take photos. .. HSC-T6 cells (CL-0116, Procell) were classified as four groups following as control group, TGF-β (80116-RNAH, SinoBiological) treatment group (48 h of 5 ng/ml TGF-β treatment), TGF-β+mimic NC group (24 h of 5 ng/ml TGF-β treatment and then 24 h of mimic NC transfection), and TGF-β + miR-125a-5p group(5 ng/ml TGF-β for 24 h, and transfected miR-125a-5p mimic (miR10000829-1-5, Ribobio) for 24 h.) Lipo RNAi MAX was used for transfection (13778-075, Thermo Fisher), and the method was carried out according to our previous paper [ ].

    Article Title: 2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis.
    Article Snippet: The stained picture was then photographed using a positive fluorescence microscope (Zeiss, Germany). .. The stained picture was then photographed using a positive fluorescence microscope (Zeiss, Germany). .. Western blot analyses Rat heart tissues or cells were lysed for protein lysate in RIPA buffer supplemented with a combination of phosphatase and protease inhibitors (CST, #5871).

    Fluorescence:

    Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy.
    Article Snippet: .. After 4% paraformaldehyde fixation, DAPI was added for 10min of nuclear staining, and a positive fluorescence microscope (Axio Imager D2, Carl Zeiss, Germany) was utilized to observe the sections and take photos. .. HSC-T6 cells (CL-0116, Procell) were classified as four groups following as control group, TGF-β (80116-RNAH, SinoBiological) treatment group (48 h of 5 ng/ml TGF-β treatment), TGF-β+mimic NC group (24 h of 5 ng/ml TGFβ treatment and then 24 h of mimic NC transfection), and TGF-β +miR125a-5p group(5 ng/ml TGF-β for 24 h, and transfected miR-125a-5p mimic (miR10000829-1-5, Ribobio) for 24 h.) Lipo RNAi MAX was used for transfection (13778-075, Thermo Fisher), and the method was carried out according to our previous paper [8].

    Article Title: Machine learning-assisted development of injectable, mechanically robust and energy metabolism-modulating brushite cements
    Article Snippet: After 2 h of incubation, a fluorescent secondary antibody (1:500, Abcam, UK) was applied to bind the primary antibodies, and DAPI was applied to stain cell nuclei. .. Finally, immunofluorescent images were acquired using a positive fluorescence microscope (Zeiss, Germany). ..

    Article Title: DNA methylation-regulated LINC02587 inhibits ferroptosis and promotes the progression of glioma cells through the CoQ-FSP1 pathway.
    Article Snippet: The paraffin-embedded brain tissues were sliced, and IHC was performed using a YN-05MY automatic immunohistochemical staining system (Shenzhen Yongnian Technology) with primary antibodies Ki67 (1:500, ab16667, Abcam, UK) and FSP1(1:500, DF863, Affinity Biosciences). .. Immunostained sections were imaged with a positive fluorescence microscope (Carl Zeiss). ..

    Article Title: Protective Effects of Isoliquiritigenin and Licochalcone B on the Immunotoxicity of BDE-47: Antioxidant Effects Based on the Activation of the Nrf2 Pathway and Inhibition of the NF-κB Pathway.
    Article Snippet: The obtained samples were analyzed through BD flow cytometry. .. The special fluorescence of the cells was observed with a Carl Zeiss positive fluorescence microscope (Oberkochen, Germany). ..

    Article Title: Arene-Arene Coupled Disulfamethazines (or Sulfadiazine)-Phenanthroline-Metal(II) Complexes were Synthesized by In Situ Reactions and Inhibited the Growth and Development of Triple-Negative Breast Cancer through the Synergistic Effect of Antiangiogenesis, Anti-Inflammation, Pro-Apoptosis, and Cuproptosis.
    Article Snippet: Cells were observed with an inverted microscope (OLYMPUS CKX35, Japan). .. Tissue sections are photographed with a positive fluorescence microscope (ZEISS, Germany). ..

    Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy
    Article Snippet: .. After 4% paraformaldehyde fixation, DAPI was added for 10 min of nuclear staining, and a positive fluorescence microscope (Axio Imager D2, Carl Zeiss, Germany) was utilized to observe the sections and take photos. .. HSC-T6 cells (CL-0116, Procell) were classified as four groups following as control group, TGF-β (80116-RNAH, SinoBiological) treatment group (48 h of 5 ng/ml TGF-β treatment), TGF-β+mimic NC group (24 h of 5 ng/ml TGF-β treatment and then 24 h of mimic NC transfection), and TGF-β + miR-125a-5p group(5 ng/ml TGF-β for 24 h, and transfected miR-125a-5p mimic (miR10000829-1-5, Ribobio) for 24 h.) Lipo RNAi MAX was used for transfection (13778-075, Thermo Fisher), and the method was carried out according to our previous paper [ ].

    Article Title: 2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis.
    Article Snippet: The stained picture was then photographed using a positive fluorescence microscope (Zeiss, Germany). .. The stained picture was then photographed using a positive fluorescence microscope (Zeiss, Germany). .. Western blot analyses Rat heart tissues or cells were lysed for protein lysate in RIPA buffer supplemented with a combination of phosphatase and protease inhibitors (CST, #5871).

    Microscopy:

    Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy.
    Article Snippet: .. After 4% paraformaldehyde fixation, DAPI was added for 10min of nuclear staining, and a positive fluorescence microscope (Axio Imager D2, Carl Zeiss, Germany) was utilized to observe the sections and take photos. .. HSC-T6 cells (CL-0116, Procell) were classified as four groups following as control group, TGF-β (80116-RNAH, SinoBiological) treatment group (48 h of 5 ng/ml TGF-β treatment), TGF-β+mimic NC group (24 h of 5 ng/ml TGFβ treatment and then 24 h of mimic NC transfection), and TGF-β +miR125a-5p group(5 ng/ml TGF-β for 24 h, and transfected miR-125a-5p mimic (miR10000829-1-5, Ribobio) for 24 h.) Lipo RNAi MAX was used for transfection (13778-075, Thermo Fisher), and the method was carried out according to our previous paper [8].

    Article Title: Machine learning-assisted development of injectable, mechanically robust and energy metabolism-modulating brushite cements
    Article Snippet: After 2 h of incubation, a fluorescent secondary antibody (1:500, Abcam, UK) was applied to bind the primary antibodies, and DAPI was applied to stain cell nuclei. .. Finally, immunofluorescent images were acquired using a positive fluorescence microscope (Zeiss, Germany). ..

    Article Title: DNA methylation-regulated LINC02587 inhibits ferroptosis and promotes the progression of glioma cells through the CoQ-FSP1 pathway.
    Article Snippet: The paraffin-embedded brain tissues were sliced, and IHC was performed using a YN-05MY automatic immunohistochemical staining system (Shenzhen Yongnian Technology) with primary antibodies Ki67 (1:500, ab16667, Abcam, UK) and FSP1(1:500, DF863, Affinity Biosciences). .. Immunostained sections were imaged with a positive fluorescence microscope (Carl Zeiss). ..

    Article Title: Protective Effects of Isoliquiritigenin and Licochalcone B on the Immunotoxicity of BDE-47: Antioxidant Effects Based on the Activation of the Nrf2 Pathway and Inhibition of the NF-κB Pathway.
    Article Snippet: The obtained samples were analyzed through BD flow cytometry. .. The special fluorescence of the cells was observed with a Carl Zeiss positive fluorescence microscope (Oberkochen, Germany). ..

    Article Title: Chlorantraniliprole Enhances Cellular Immunity in Larvae of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)
    Article Snippet: .. The slices were then placed in a humidor, incubated at 28 °C for 30 min, and observed under the positive-fluorescence microscope (Axio ImagerZ2, Zeiss, Oberkochen, Germany). ..

    Article Title: Arene-Arene Coupled Disulfamethazines (or Sulfadiazine)-Phenanthroline-Metal(II) Complexes were Synthesized by In Situ Reactions and Inhibited the Growth and Development of Triple-Negative Breast Cancer through the Synergistic Effect of Antiangiogenesis, Anti-Inflammation, Pro-Apoptosis, and Cuproptosis.
    Article Snippet: Cells were observed with an inverted microscope (OLYMPUS CKX35, Japan). .. Tissue sections are photographed with a positive fluorescence microscope (ZEISS, Germany). ..

    Article Title: Overexpression miR-125a-5p inhibits HSCs activation and alleviates liver fibrosis through TGF-β/Smad2/3 signaling pathway and autophagy
    Article Snippet: .. After 4% paraformaldehyde fixation, DAPI was added for 10 min of nuclear staining, and a positive fluorescence microscope (Axio Imager D2, Carl Zeiss, Germany) was utilized to observe the sections and take photos. .. HSC-T6 cells (CL-0116, Procell) were classified as four groups following as control group, TGF-β (80116-RNAH, SinoBiological) treatment group (48 h of 5 ng/ml TGF-β treatment), TGF-β+mimic NC group (24 h of 5 ng/ml TGF-β treatment and then 24 h of mimic NC transfection), and TGF-β + miR-125a-5p group(5 ng/ml TGF-β for 24 h, and transfected miR-125a-5p mimic (miR10000829-1-5, Ribobio) for 24 h.) Lipo RNAi MAX was used for transfection (13778-075, Thermo Fisher), and the method was carried out according to our previous paper [ ].

    Article Title: 2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis.
    Article Snippet: The stained picture was then photographed using a positive fluorescence microscope (Zeiss, Germany). .. The stained picture was then photographed using a positive fluorescence microscope (Zeiss, Germany). .. Western blot analyses Rat heart tissues or cells were lysed for protein lysate in RIPA buffer supplemented with a combination of phosphatase and protease inhibitors (CST, #5871).

    Incubation:

    Article Title: Chlorantraniliprole Enhances Cellular Immunity in Larvae of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)
    Article Snippet: .. The slices were then placed in a humidor, incubated at 28 °C for 30 min, and observed under the positive-fluorescence microscope (Axio ImagerZ2, Zeiss, Oberkochen, Germany). ..



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